Studies on the Enzyme-Substrate Complex. II
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چکیده
منابع مشابه
Kinetic studies on the enzyme-substrate complex formation of p-hydroxybenzoate hydroxylase by the stopped-flow method.
The spectrophotometric and spectrofiuorometric investigations of the enzyme-substrate complex formation of p-hydroxybenzoate hydroxylase was made by the stopped-flow technique. The apparent velocity of the formation of the enzyme-substrate complex (the velocity of the absorbance change in visible and UV regions, and the velocity of the quenching of the fluorescence intensity in the FAD moiety o...
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The stability constants of species present in the systems Ni(II)-pyridoxamine(pym)(A) and Ni(II)-pyridoxamine(pym)(A)-imidazole containing ligands(B) [B = imidazole(him), benzimidazole(bim), histamine(hist) and L-histidine(his)] have been determined pH-metrically using the MINIQUAD computer program. The existence of the species NiAH, NiA and NiA2 was proven for the Ni(II)-pym(A)...
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The photoenzyme from bakers' yeast which repairs ultravioletinactivated transforming DNA is mechanically bound to ultraviolet-irradiated DNA in the dark, but not to unirradiated DNA. In the bound condition it is stabilized against inactivation by heat and heavy metals. Both the mechanical binding and stabilization are eliminated by illumination. These observations are consistent with the reacti...
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p-Hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas desmolytica is known to form a stable complex with its substrate. Investigations were made on properties of the complex by spectrophotometric measurements and photooxidation. The addition ofp-hydroxybenzoate or benzoate to the oxidized (with respect to bound FAD) enzyme solution caused changes in absorption, fluorescence, and CD spec...
متن کاملBio-Inorganic Studies on the Fe(II) Sparfloxacin Complex
The qualitative and quantitative analysis of an antibiotic drug, 5-amino-1 cyclopropyl-7 (cis-3, 5 dimethyl-1-piperazyl)-6,8- dihydro-1, 4 dihydro-4-oxo-3-quinoline carboxylic acid (Sparfloxacin, SFX) and its pharmaceutical formulation i.e.sparx-100 tablet, has been done using polarographic and amperometric methods. Complexation behavior of SFX with Fe(II), both in solid and liquid phases has b...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1960
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.7.64